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KMID : 0614620100560010027
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Korean Journal of Gastroenterology
2010 Volume.56 No. 1 p.27 ~ p.32
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Culture and Polymerase Chain Reaction of Helicobacter pylori from Rectal and Terminal Ileal Fluid after Polyethylene Glycol (Colyte®) Ingestion in Healthy Adults with Positive Urea Breath Test
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Kim Do-Hyeon
Jung Hong-Myong
Hwang Young-June
Ahg Yong-Soo
Mun Jang-Sik
Myoung Bo-Hyun
Park Hyuk
Jeong Eun-Joo
Im Yun-Mi
Oh Hyun-Min
Jeong Hui-Yeong
Park Chul
Kim Hyung-Rag
Cho Eun-Hae
Kim Ho-Dong
Jung Young-Do
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Abstract
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Background/Aims: Helicobacter pylori (H. pylori) transmission route is not yet clearly understood. Isolating H. pylori from stool, saliva, and vomitus is very difficult. However, H. pylori could be cultured from feces in the setting of rapid gastrointestinal tract transit. The aim of this study was to isolate H. pylori by culture and PCR in the rectum and terminal ileum during colonoscopy.
Methods: Twenty subjects with positive UBT (urea breath test) were included. We performed polymerase chain reaction (PCR) test and culture of H. pylori with the rectal fluid and terminal ileal fluid during colonoscopy.
Results: H. pylori was cultured with rectal fluid from 9 (45.0%) of 20 subjects and with ileal fluid from 11 (55.0%) of 20 subjects. H. pylori was a little more frequently cultured from the terminal ileal fluid than the rectal fluid without statistical significance (p>0.05). PCR test detected flaA (16/20, 80.0% and 17/20, 85.0%), 16S rRNA gene (16/20, 80.0% and 17/20, 85.0%), cagA (10/20, 50.0% and 12/20, 60.0%), and ureC (9/20, 45% and 11/20, 54.5%) from the rectal fluid and the terminal ileal fluid, respectively. The specificity and sensitivity of ureC were 100%.
Conclusions: H. pylori could be cultured from the rectal fluid and terminal ileal fluid in the setting of rapid gastrointestinal tract transit. These results suggest of fecal-oral transmission of H. pylori.
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KEYWORD
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Urea breath test, Helicobacter pylori, Polyethylene glycol, H. pylori DNA (flaA, 16S rRNA gene and cagA)
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